Screening of Actinomycetes for Asparaginase Production and it’s Optimization Conditions

The enzyme Asparaginase, known as L-asparagine amidohydrolase, has anti-tumor properties and is widely used a chemotherapeutic treatment for acute lymphoblastic leukemia. Many different organisms, including plants terrestrial microbes such bacteria, fungi, algae actinomycetes, have produced this enzyme. In study, 34 isolates of the actinomycetes were collected from soil water governorates in Egypt. Only nine Streptomyces identified by presence pink hue around their colonial growth an indication had best ability synthesis extracellular Asparaginase. Glucose peptone effective carbon nitrogen sources, respectively. ideal pH Asparaginase 7, temperature 30